Determination of Significance：

Superoxide dismutase (SOD, EC 1.15.1.1) is widely found in animals, plants, microorganisms and cultured cells. SOD represents a group of enzymes that use as cofactor copper and zinc, or manganese, or iron ions. Cu/Zn SOD is located in the cytoplasm, and Mn SOD in the mitochondria. It catalyzes the superoxide anion to form H₂O₂ and O2. SOD is not only the superoxide anion scavenging enzyme, but also the main H₂O₂ producing enzyme, which plays an important role in the biological antioxidant system.

Measurement Principle：

Superoxide anion (O^2-) is produced by xanthine and xanthine oxidase reaction system. O^2- can reduce water-soluble tetrazolium-1(WST-1) to form a yellow formazan dye, which has absorbance in 450 nm. SOD can remove O^2- and inhibit the formation of the formazan dye. The darker the yellow color of the reaction solution, the lower the SOD activity. The lighter the yellow color of the reaction solution, the higher the activity of SOD. Cu/Zn SOD activity unchanged and Mn SOD activity inactivated after treated. Mn SOD activity could be calculated by determination of total SOD activity and Mn SOD activity.

Self Provided：

Spectrophotometer, table centrifuge, constant temperature foster box/water-bath, vortex mixer/oscillator, water bath, transferpettor, 1mL glass cuvette, mortar/ homogenizer/cell ultrasonic crusher, ice and sterilized water.

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