Plant Root Vitality （TTC-Method） Assay Kit
Cat.No：BC5275 Solarbio
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at 2-8℃，6 months
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> Biochemical Assay Kits > Other Biochemical Series > Other Series > Plant Root Vitality （TTC-Method） Assay Kit
Product Information
| Reagents Required But Not Provided | Please check the manual first, there are some reagents you should prepare by yourself. |
| Name | Plant Root Vitality （TTC-Method） Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8℃，6 months |
| Recommend | If the 96-well plate is used, the polystyrene material is not used, and the 96-well quartz plate is recommended |
| Unit | Kit |
| Specification | 100T/48S |
Determination of Significance：
The root is the main organ for plants to absorb water and mineral nutrients, and it is also the organ for the synthesis, assimilation and transformation of important substances in plants such as amino acids and hormones. Therefore, the growth and activity of roots directly affect the growth of individual plants. Nutrient level and yield level, etc. Root vigor has important practical significance.
Measurement Principle：
TTC can be reduced to insoluble red triphenylformazan (TTF) by hydrogen, and TTF has an absorption peak at 485 nm. When the TTC solution penetrates into the root tissue of the plant, the reducing substances produced by the respiration process can reduce it to TTF (red), and the root tissue of the plant is stained red. The root vigor can be expressed by the reduction amount of TTC. The root activity detected by this method is the dehydrogenase activity of plant roots.
Self Provided：
Spectrophotometer/microplate reader, centrifuge, water bath/incubator, adjustable pipette, micro glass cuvettes/96-well plates (non-polystyrene), mortar/homogenizer, ethyl acetate, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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