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Determination of Significance：
S-NR catalyzes the reduction of nitrate to nitrite in soil, which is the key enzyme of nitrate reduction in soil. Study on the activity of S-NR is of great significance for rational fertilization and reduction of nitrogen loss.
Measurement Principle：
S-NR catalyzes the reduction of nitrate to nitrite, NO3-+NADH+H+→NO2-+NAD++H2O; the generated nitrite can quantitatively generate red azo compounds with p-aminobenzenesulfonic acid and α-naphthylamine under acidic conditions; The unreacted NADH will inhibit the subsequent color reaction, and then carry out the subsequent reaction with PMS; the generated red azo compounds are 520 nm has a maximum absorption peak, which can be determined by spectrophotometry.
Self Provided：
Spectrophotometer/microplate reader, water bath, centrifuge, adjustable pipette, 30 mesh sieve (or smaller), micro glass cuvette/96 well flat-bottom plate, ice and distilled water.
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