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Soil Nitrate Reductase(S-NR)Activity Assay Kit

BC3105-241212Manual

Cat.No：BC3105 Solarbio

Detection Method：Spectrophotometer/Microplate Reader

Storage：Store at -20℃，6 months

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Product Information

Name	Soil Nitrate Reductase(S-NR)Activity Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Unit	Kit
Specification	100T/48S

Determination of Significance：

S-NR catalyzes the reduction of nitrate to nitrite in soil, which is the key enzyme of nitrate reduction in soil. Study on the activity of S-NR is of great significance for rational fertilization and reduction of nitrogen loss.

Measurement Principle：

S-NR catalyzes the reduction of nitrate to nitrite, NO^3-+NADH+H+→NO^2-+NAD++H2O; the generated nitrite can quantitatively generate red azo compounds with p-aminobenzenesulfonic acid and α-naphthylamine under acidic conditions; The unreacted NADH will inhibit the subsequent color reaction, and then carry out the subsequent reaction with PMS; the generated red azo compounds are 520 nm has a maximum absorption peak, which can be determined by spectrophotometry.

Self Provided：

Spectrophotometer/microplate reader, water bath, centrifuge, adjustable pipette, 30 mesh sieve (or smaller), micro glass cuvette/96 well flat-bottom plate, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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