Determination of Significance:
The activity of plant dehydrogenase (PDHA) is largely reflects the active state of the organism, which can directly indicate the ability of biological cells to degrade its matrix.
Measurement Principle:
The hydrogen acceptor 2,3,5-triphenyl tetrazolium chloride (TTC) generates red triphenylformazone (TFF) after receiving hydrogen during cell respiration. TFF has a characteristic absorption peak at 485 nm, the PDHA activity can quantified by measuring the absorbance at 485 nm.
Self Provided:
Microplate Reader or spectrophotometer, water bath, desk centrifuge, water bath, pipette, micro glass cuvette/96 well flat-bottom plate (non-polystyrene/polypropylene material), mortar/homogenizer, ethyl acetate (express delivery is not allowed), ice and distilled water.
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