Determination of Significance:
Betaine is a kind of quaternary ammonium type water-soluble alkaloid widely distributed in animals, plants and microorganisms. It is the oxidation product of choline in organism. It can enhance immunity, reduce blood lipid, resist oxidation and anti-tumor. It can also be used as a methyl donor to promote protein and fat metabolism, increase appetite, relieve stress, regulate osmotic pressure, stabilize vitamins and other biological functions It is widely used in chemical industry, medicine, food additive and other fields.
Measurement Principle:
Under strong acid conditions, betaine reacts with Raynaud salt to produce precipitation. The precipitation is dissolved in acetone to form a red solution. There is a characteristic absorption peak at 525 nm. The absorption value at 525 nm is determined to obtain the content of betaine in the sample.
Self Provided:
Table type centrifuge, spectrophotometer/microplate reader, water-bath, micro glass cuvette/96 well flat-bottom plate, transferpettor, mortar/homogenizer, methanol, ether, HCl, distilled water.
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可以检测动物组织里含量吗,检测灵敏度及范围是多少2022-07-18 16:36:17
Administrator可以检测动物组织;最低检出限1mg/mL,线性范围2—9mg/mL。