Phytase Activity Assay Kit

Determination of Significance：

Phytase (Phytase), is a binding enzyme of protein and sugar. Phytase can decompose phytic acid to produce inorganic phosphorus and inositol, which greatly improves the utilization rate of nutrients by organisms. Phytase widely exists in plants, animal tissues and microorganisms. Now, microorganisms are used to synthesize phytase for production and application. Phytase has extensive research value in the fields of food production and animal husbandry.

Measurement Principle：

Under certain environmental conditions, phytase can decompose sodium phytate to generate inorganic phosphorus and inositol derivatives. Under acidic conditions, inorganic phosphorus and ammonium molybdate chromogen react. A blue molybdenum blue substance is produced, which has a characteristic absorption peak at 700 nm, and the activity of phytase can be calculated by measuring the content of inorganic phosphorus.

Self Provided：

Spectrophotometer/microplate reader, Table Centrifuge, Water-bath/Constant Temperature Incubator, Micro Quartz Cuvette/96 Well UV Flat-bottom Plate, Mortar/Homogenate/Cell ultrasonic Crusher, Ultrasonic Dissolver, Gyrotron Oscillator, Ice and Distilled Water, Concentrated sulfuric acid.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.