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Modified Sirius Red Stain Kit(No Picric Acid)

Storage:Store at RT,avoid light,1 year

Modified Sirius Red Stain Kit(No Picric Acid)
Cat.No:
G1472
Brand:
Solarbio

Product Introduction:

Collagen fiber is the most widely distributed and abundant fiber in connective tissue. It is widely distributed in various organs. Type I collagen fibers are mainly bone, skin and tendon; Type Ⅱ collagen fibers are mainly cartilage collagen; Type Ⅲ collagen fibers are mainly found in embryonic tissues, adult blood vessels and gastrointestinal tract; Type Ⅳ collagen fibers are mainly in the basement membrane. Sirius red and its lining dye solution are strong acid dyes, which are easy to combine with the basic groups in collagen molecules and have strong adsorption. Polarized light microscopy shows that the collagen fibers have the property of positive uniaxial birefringence. When combining with Sirius red compound staining solution, it could enhance the birefringence and improve the resolution, so as to distinguish the two types of collagen fibers.
Modified Sirius Red Stain Kit(No Picric Acid) is mainly composed of Iron Hematoxylin Stain and Sirius Red Stain, without picric acid. It is mainly used in the study of collagen fiber abnormality or fiber proliferation in various tissue diseases. Under the ordinary optical microscope, the collagen fibers of heart and blood vessels and other tissues are dyed red, which is helpful for the classification and grading of various fibrotic diseases under the polarized light microscope. Immunohistochemical technique can also display type Ⅰ and Ⅲ collagen fibers, but the antibodies used are expensive and time-consuming, while Sirius red staining reagent is cheap and easy to operate.


Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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