Storage:Store at RT,avoid light,1 year
Product Introduction:
Collagen fiber is the most widely distributed and abundant fiber in connective tissue. It is widely distributed in various organs. Type I collagen fibers are mainly bone, skin and tendon; Type Ⅱ collagen fibers are mainly cartilage collagen; Type Ⅲ collagen fibers are mainly found in embryonic tissues, adult blood vessels and gastrointestinal tract; Type Ⅳ collagen fibers are mainly in the basement membrane. Sirius red and its lining dye solution are strong acid dyes, which are easy to combine with the basic groups in collagen molecules and have strong adsorption. Polarized light microscopy shows that the collagen fibers have the property of positive uniaxial birefringence. When combining with Sirius red compound staining solution, it could enhance the birefringence and improve the resolution, so as to distinguish the two types of collagen fibers.
Modified Sirius Red Stain Kit(No Picric Acid) is mainly composed of Iron Hematoxylin Stain and Sirius Red Stain, without picric acid. It is mainly used in the study of collagen fiber abnormality or fiber proliferation in various tissue diseases. Under the ordinary optical microscope, the collagen fibers of heart and blood vessels and other tissues are dyed red, which is helpful for the classification and grading of various fibrotic diseases under the polarized light microscope. Immunohistochemical technique can also display type Ⅰ and Ⅲ collagen fibers, but the antibodies used are expensive and time-consuming, while Sirius red staining reagent is cheap and easy to operate.
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请问冰冻切片可以做天狼星红染色吗2023-05-24 09:52:57
Administrator您好,可以做冰冻切片染色,冰冻切片通常较厚,可能需要增加染色时间,建议根据实际染色情况调整染色时间。
请问用什么软件分析胶原1和3的定量呢2022-08-02 17:48:39
Administrator您好,用偏光显微镜观察可以定性观察区分,定量分析没有做过了,您如果实验涉及到这一方面再查一下相关文献吧
改良版天狼星红染色可以用于肺纤维化染色吗?有在文献中验证过吗?天狼星红染色和Van Gieson染色有区别吗?2022-07-19 17:45:53
Administrator1. 本产品可用于各种组织病变时胶原纤维异常或纤维增生的染色。
2. 在配方上有所不同,Van Gieson氏染色液中含有苦味酸,本品中不含。经本品染色,胶原纤维在偏光镜下呈现双折光,Ⅰ型胶原纤维呈强橙黄色或亮红色,Ⅲ型胶原纤维呈绿色。