Transhydrogenase-2 Activity Assay Kit
Cat.No：BC3265 Solarbio
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6 months
Qty：
-
+
Size：
{{cart_num}}
My CartTel: +86 010 50973105
Products
Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Your Position: Home
> Biochemical Assay Kits > Protein ＆ Enzyme Series > Transhydrogenase Series > Transhydrogenase-2 Activity Assay Kit
Product Information
| Name | Transhydrogenase-2 Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/48S |
Determination of Significance：
Transhydrogenase (TH) is located in the inner membrane of mitochondria, also as known as Mitochondrial Respiratory Chain Complex VI. The enzyme catalyzes the mutual conversion of NADH+NADP+ and NAD++NADPH, and regulate the balance between NAD (H) and NADP (H) in mitochondria. The reverse reaction is called TH-2, which catalyzes NADPH and NAD+ to generate NADP+ and NADH.
Measurement Principle：
Both NADH and NADPH have characteristic absorption peak at 340 nm, therefore, the hydrogen transfer reaction catalyzed by TH cannot has a absorbance change at 340 nm. Replaces NAD+ with 3-acetylpyridyl adenine dinucleotide (APAD+, synthetic substrate), TH-2 can catalyzes APAD+ reduction to APADH and the reaction product has a characteristic absorption peak at 375 nm. In this kit, the TH-2 activity is quantified by measuring the rate of increase in light absorption at 375 nm.
Self Provided：
Spectrophotometer/microplate reader, water bath, desk centrifuge, water bath, adjustable transferpettor, micro quartz cuvette/96 well plate (96 UV plate), mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Sorry, there is no experimental images.
Related Products
Sorry, there is no more information.
Related Paper
Sorry, there is no more information.
Technical Q&A
Q&A
（Total 0
User Q&A）
- There is not question and answer now, come and send an inquiry please.
My question
Service for Scientific Research, Responsible for the Life Science
Contact Us
Address: No 85, Liandong U Valley, Middle Zone, 101102, Tongzhou Dist,
Beijing, China
Tel: +86 010 50973105
Email: info@solarbio.com
Order Guide
Service Guarantee
Resources Download
Copyright © 2005-2021 Solarbio all rights reserved.