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Storage:Store at -20℃，6 months
Determination of Significance：
Transhydrogenase (TH) is located in the inner membrane of mitochondria, also as known as Mitochondrial Respiratory Chain Complex VI. The enzyme catalyzes the mutual conversion of NADH+NADP+ and NAD++NADPH, and regulate the balance between NAD (H) and NADP (H) in mitochondria. The reverse reaction is called TH-2, which catalyzes NADPH and NAD+ to generate NADP+ and NADH.
Measurement Principle：
Both NADH and NADPH have characteristic absorption peak at 340 nm, therefore, the hydrogen transfer reaction catalyzed by TH cannot has a absorbance change at 340 nm. Replaces NAD+ with 3-acetylpyridyl adenine dinucleotide (APAD+, synthetic substrate), TH-2 can catalyzes APAD+ reduction to APADH and the reaction product has a characteristic absorption peak at 375 nm. In this kit, the TH-2 activity is quantified by measuring the rate of increase in light absorption at 375 nm.
Self Provided：
Spectrophotometer/microplate reader, water bath, desk centrifuge, water bath, adjustable transferpettor, micro quartz cuvette/96 well plate (96 UV plate), mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
