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Storage:Store at -20℃，6 months
Determination of Significance：
PEPCK (EC 4.1.1.32) is widely found in animals, flowering plants, algae, some fungi and bacteria. The enzyme catalyzes the conversion of oxaloacetic acid to phosphoenolpyruvate, which is the first-rate limiting enzyme regulating gluconeogenesis.
Measurement Principle：
PEPCK catalyzes oxaloacetic acid to form phosphoenolpyruvate and CO2, pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to NAD+ in turn, and determine the NADH decline rate at 340 nm, which can reflect the PEPCK activity.
Self Provided：
Ultraviolet spectrophotometer/microplate reader, low temperature centrifuge, water bath pot, micro quartz cuvette/96 well flat-bottom plate (UV), adjustable pipette, mortar/homogenizer, ice and distilled water
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
