{{cart_num}}
My Cart> Biochemical Assay Kits > Carbohydrate Metabolism Series > Gluconeogenic Series > Phosphoenolpyruvate Carboxykinase(PEPCK) Activity Assay KitDetection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6 months
Qty：
Size：
| Name | Phosphoenolpyruvate Carboxykinase(PEPCK) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
PEPCK (EC 4.1.1.32) is widely found in animals, flowering plants, algae, some fungi and bacteria. The enzyme catalyzes the conversion of oxaloacetic acid to phosphoenolpyruvate, which is the first-rate limiting enzyme regulating gluconeogenesis.
Measurement Principle：
PEPCK catalyzes oxaloacetic acid to form phosphoenolpyruvate and CO2, pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to NAD+ in turn, and determine the NADH decline rate at 340 nm, which can reflect the PEPCK activity.
Self Provided：
Ultraviolet spectrophotometer/microplate reader, low temperature centrifuge, water bath pot, micro quartz cuvette/96 well flat-bottom plate (UV), adjustable pipette, mortar/homogenizer, ice and distilled water
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no experimental images.
Sorry, there is no more information.
Sorry, there is no more information.
