Glycogen synthase(GCS) Activity Assay Kit
Cat.No：BC3335 Solarbio
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Glycometabolic Series > Glycogen synthase(GCS) Activity Assay Kit
Product Information
| Name | Glycogen synthase(GCS) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Glycogen synthetase (GCS) can add the glycogen of UDPG to the original glycogen or the non-reducing end of glycogen protein, and be connected by α-1,4 glycoside bond. GCS is the rate limiting enzyme of glycogen synthesis in animal body. It is also the main target enzyme of insulin. It plays an important role in the process of glucose metabolism and maintaining the relative stability of blood glucose.
Measurement Principle：
GCS catalyzes the production of glycogen and UDP from UDPG and glucose residues. Pyruvate kinase and lactate dehydrogenase further catalyze NADH to generate NAD+. The decrease rate of NADH at 340 nm can reflect the activity of GCS.
Self Provided：
Ultraviolet spectrophotometer/microplate reader, balance, low temperature desk centrifuge, water-bath, transferpettor, micro quartz cuvette/96 well flat-bottom UV plate, EP tube, mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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