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Determination of Significance：
Glycogen synthetase (GCS) can add the glycogen of UDPG to the original glycogen or the non-reducing end of glycogen protein, and be connected by α-1,4 glycoside bond. GCS is the rate limiting enzyme of glycogen synthesis in animal body. It is also the main target enzyme of insulin. It plays an important role in the process of glucose metabolism and maintaining the relative stability of blood glucose.
Measurement Principle：
GCS catalyzes the production of glycogen and UDP from UDPG and glucose residues. Pyruvate kinase and lactate dehydrogenase further catalyze NADH to generate NAD+. The decrease rate of NADH at 340 nm can reflect the activity of GCS.
Self Provided：
Ultraviolet spectrophotometer/microplate reader, balance, low temperature desk centrifuge, water-bath, transferpettor, micro quartz cuvette/96 well flat-bottom UV plate, EP tube, mortar/homogenizer, ice and distilled water.
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