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Determination of Significance：
Glycogen phosphorylase is divided into active glycogen phosphorylase a (Glycogen phosphorylase a, GPa) and inactive glycogen phosphorylase b (Glycogen phosphorylase b, GPb) two forms. The decomposition of glycogen is mainly carried out under the catalysis of glycogen phosphorylase a. When no activator is added, glycogen phosphorylase a catalyzes the production of glucose residues from glycogen and inorganic phosphorus to glycogen and glucose 1-phosphate. Under the action of phosphoglucose mutase and 6-phosphate glucose dehydrogenase, it further catalyzes the reduction of NADP to NADPH. Measuring the rate of increase of NADPH at 340nm can reflect the activity of glycogen phosphorylase a. The activity of GP (GPa and GPb) is measured by adding an activator, and GPa activity is measured when no activator is added. GPb activity can be obtained by subtracting GPa activity from GP activity.
Measurement Principle：
Glycogen phosphorylase is divided into active glycogen phosphorylase a (Glycogen phosphorylase a, GPa) and inactive glycogen phosphorylase b (Glycogen phosphorylase b, GPb) two forms. The decomposition of glycogen is mainly carried out under the catalysis of glycogen phosphorylase a. When no activator is added, glycogen phosphorylase a catalyzes the production of glucose residues from glycogen and inorganic phosphorus to glycogen and glucose 1-phosphate. Under the action of phosphoglucose mutase and 6-phosphate glucose dehydrogenase, it further catalyzes the reduction of NADP to NADPH. Measuring the rate of increase of NADPH at 340nm can reflect the activity of glycogen phosphorylase a. The activity of GP (GPa and GPb) is measured by adding an activator, and GPa activity is measured when no activator is added. GPb activity can be obtained by subtracting GPa activity from GP activity.
Self Provided：
Ultraviolet spectrophotometer/microplate reader, low temperature centrifuge, constant temperature incubator/water bath, adjustable pipette, mortar/homogenizer, micro quartz cuvette/96 well UV flat -bottom plate, ice and distilled water.
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