UDP-glucose pyrophosphosphprylase(UGP) Activity Assay Kit
Cat.No：BC3365 Solarbio
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Glycometabolic Series > UDP-glucose pyrophosphosphprylase(UGP) Activity Assay Kit
Product Information
| Name | UDP-glucose pyrophosphosphprylase(UGP) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
UDP-glucose pyrophosphorylase (UDP-glucose pyrophosphosphprylase, UGP, EC2.7.7.9) is widely distributed in nature. It catalyzes the activation of glucose before glycogen synthesis. UDP-glucose (UDPG) is synthesized from glucose-1-phosphate and UTP. UDPG is the main active enzyme form in higher plants and animals. As a glucose-based donor, it participates in the synthesis and metabolism of glycogen, sucrose, cellulose, etc.
Measurement Principle：
UGP can catalyze the reversible formation of glucose-1-phosphate. NADP was transformed into NADPH by phosphoglucose mutase and 6-phosphoglucose dehydrogenase. UGP activity can be reflected by the change of 340nm absorption value.
Self Provided：
Ultraviolet spectrophotometer/microplate reader, balance, low temperature desk centrifuge, water-bath, transferpettor, micro quartz cuvette/96-well flat-bottom UV plate, EP tube, mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Related Paper
The SnRK2.3-AREB1-TST1/2 cascade activated by cytosolic glucose regulates sugar accumulation across tonoplasts in apple and tomato
Click to check >>Author：Zhu Lingcheng, Li Yanzhen, Wang Chengcheng, Wang Zhiqi, Cao Wenjing, Su Jing, Peng Yunjing, Li Baiyun, Ma Baiquan, Ma Fengwang, Ruan Yong-Ling, Li Mingjun
IF：18.0000
Publish_to：Nature Plants
PMID：37291399
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