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My Cart> Biochemical Assay Kits > Carbohydrate Metabolism Series > Photosynthetic Series > Pyrophosphate: Fructose-6-Phosphate-1-Phosphoric Acid Transferase(PFP) Activity Assay KitDetection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6 months
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| Name | Pyrophosphate: Fructose-6-Phosphate-1-Phosphoric Acid Transferase(PFP) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Pyrophosphate: Fructose-6-phosphate-1-phosphotransferase (PFP, EC2.7.1.90) is a cytosolic enzyme that is widely present in plant tissues and catalyzes the phosphorylation of fructose-6-phosphate like phosphofructokinase. As a result, the single PEP catalytic reaction is a reversible reaction, and pyrophosphate is used instead of ATP, which plays an important role in carbon metabolism of photosynthesis.
Measurement Principle：
PFP catalyzes the conversion of fructose 6-phosphate to fructose 1,6-diphosphate, which is converted to dihydroxyacetone phosphate by the action of aldolase and triose phosphate isomerase, and then catalyzed by α-phosphate glycerol dehydrogenase and NADH to from Glycerol 3-diphosphate and NAD. The change in absorbance at 340 nm reflects the level of PFP activity.
Self Provided：
Low temperture centrifuge, spectrophotometer/microplate reader, water bath/constant temperature incubator, mortar/homogenizer, micro quartz cuvette/96 well plate, transferpettor, ice and distilled water, EP tube.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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