Glycyrrhizic acid

Examples of using this product（for reference only）

LC-MS

The samples were analyzed by LC-MS system . A 10-ml solution was injected into the LC column (C18 2.6μm 100 ×2.1 mm) with a flow rate of 0.3 ml/min. The column temperature was 4 ℃. Buffer A consisted of 0.1% formic acid in the water, and Buffer B was methanol. The gradient was 5% Buffer B for 1 min,5–95% Buffer B for 14 min, and 95% Buffer B for 3 min.The sample was analyzed by mass spectrometer with directinfusion of the sample into the electrospray ionization source operating in the negative ion mode. Curtain gas, ion source gas and auxiliary gas were 40 psi, 65 psi and 65 psi, respectively.IonSpray voltage floating, collision energy and declustering potential were set to -4500 V, -10 V and -80 V, respectively,with a gasification temperature of 550 ℃.

References：

Song M, Lei Y, Ali A, Xu Y, Sheng K, Huang T, Huang J, Huang M. Inhibitory effect of licorice extract on the germination and outgrowth of Paraclostridium bifermentans spores. Front Microbiol. 2022 Dec 2;13:1076144. doi: 10.3389/fmicb.2022.1076144. PMID: 36532483; PMCID: PMC9755857

UPLC-PDA

The levels of the main active ingredients of MSJZT were detected using a UPLC-PDA method. Chromatographic separation was performed on a UPLC system with a photodiode array detector (PDA) and C18 (100 mm×2.1 mm, 1.7μm ) column. The column temperature was set at 30℃. The mobile phase was composed of 0.1% phosphoric acid (A) and acetonitrile (B). The optimum adsorption and elution conditions were set as follows: 0–1.5 min, 95% B; 1.5–6 min, 95–80% B; 7–10 min, 80–60% B; 10–10.5 min, 50–35% B; 10.5–12 min, 35–30% B; 12–12.5 min, 30–20% B; 12.5–16 min, 20–10% B; 16–16.5 min, 10–0% B; and 16.5–18 min, 0–95% B.

References：

Nie S, Zhao Y, Qiu X, Wang W, Yao Y, Yi M, Wang D. Metabolomic Study on Nude Mice Models of Gastric Cancer Treated with Modified Si Jun Zi Tang via HILIC UHPLC-Q-TOF/MS Analysis. Evid Based Complement Alternat Med. 2019 Jun 23;2019:3817879. doi: 10.1155/2019/3817879. PMID: 31341492; PMCID: PMC6612382.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.