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Determination of Significance：
Pyruvate plays an important role in the metabolism of glucose, fatty acids and amino acids through acetyl CoA.
Measurement Principle：
At pH=7.5, pyruvate reacts with NADH catalyzed by LDH to produce NAD+ and lactic acid. Under 1-mPMS, WST-1 reacts with NADH to produce water-soluble Formazan. Pyruvate content can be calculated by detecting the absorption value at 450nm.
Self Provided：
Ultraviolet spectrophotometer, desk centrifuge, water-bath/ constant temperature incubator, adjustable pipette, transferpettor, mortar/homogenizer, 1 mL glass cuvette, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：
These protocols are for reference only. Solarbio does not independently validate these
methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
