Determination of Significance：

High-density lipoprotein (HDL) is a lipoprotein that contains a small amount of cholesterol and carries cholesterol away from body cells and tissues. High-density lipoprotein cholesterol (HDL-C) concentrations negatively correlate with the incidence of atherosclerosis and coronary heart disease. Therefore, accurate and precise measurements of patients’ HDL-C concentrations are necessary to appropriately identify individuals with atherosclerosis, coronary heart disease and hypertension.

Measurement Principle：

Cholesterol is specifically dissociated by one surfactant from HDL. Esterase can catalyze the hydrolysis of cholesterol ester to produce free cholesterol (FC) and free fatty acid (FFA), thus transforming cholesterol ester into FC; Furthermore, cholesterol oxidase can catalyze FC to form Δ4-cholesterone and H₂O₂; Finally, peroxidase can catalyze the oxidation of 4-aminoantipyrine and phenyl amines by H₂O₂ to form purple quinones. It has a characteristic absorption peak at 546 nm, and its color depth is directly proportional to cholesterol content.

Self Provided：

Spectrophotometer/microplate reader, balance, low temperature table centrifuge, constant temperature incubator/water bath, micro glass cuvette/96 well flat-bottom plate, pipette, mortar/homogenizer/cell ultrasonic crusher, ice, distilled water, isopropyl alcohol.

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