Product Introduction:

In the process of tissue sectioning, when some tissues contain bone or calcification, the tissue containing calcium should not be directly embedded in paraffin. This is because the density between calcium and paraffin is different, it is difficult to cut a complete section. It is better to fix the calcium containing tissue before decalcification or both operate at the same time. Then continue next operations such as dehydration, transparency, wax immersion, embedding and slicing.There are many reagents used for decalcification. Decalcification agents include organic and inorganic acids as well as electrolytic decalcification.

During the decalcification process, the degree of decalcification should be measured at regular intervals to avoid excessive decalcification which may increase the degree of tissue damage and affect the staining results. The Decalcification Endpoint Assay (Chemical Method) is a rapid test to determine if decalcification is complete and without physical damage to the decalcified tissue.

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