Acid-Fast Bacillus(AFB)Stain Kit(Modified Kinyoun’s Method)

Product Introduction:

The cell wall of acid fast bacteria (such as Mycobacterium, Mycobacterium tuberculosis, Nocardia, etc.) contains a large amount of lipids surrounding the outside of peptidoglycans, so acid fast bacteria are not colored or lightly colored in general bacterial staining solution. The traditional dyeing method requires heating and extending the dyeing time to promote its coloring. Once the branching acid in the cell wall of acid fast bacteria combines with dyes, it is difficult to be decolorized by acidic decolorization solution, hence the name acid-fast staining.The most representative one is the Ziehl-Neelsen staining method for Mycobacterium tuberculosis, which is recommended by WHO for thermal staining.

The Acid-Fast Bacillus(AFB)Stain Kit(Modified Kinyoun’s Method) belongs to cold staining solution and does not require heating, making it relatively safer than acid-fast hot staining solution. The staining principle is that under room temperature conditions, mycobacterial acid combines with fuchsin to form a complex. After re-dyeing with methylene blue, mycobacteria still turn red, while other bacteria and substances in the background turn blue. This staining solution is slightly shallower than the Ziehl-Nielsen staining method and more suitable for staining against weak acid bacteria.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.