SYBR Green I (PCR Grade, 10000X)
Cat.No：SR4110 Solarbio
Storage：Store at -20℃，avoid light，1 year.
Appearance：Liquid
Qty：
-
+
Size：
{{cart_num}}
My CartTel: +86 010 50973105
Products
Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
| Name | SYBR Green I(PCR Level，10000X) |
| Appearance | Liquid |
| Storage | Store at -20℃，avoid light，1 year. |
| Unit | Piece |
| Specification | 50ul 100ul 1ml |
Product Description:
SYBR Green I dye is a fluorescent dye that binds directly to double-stranded DNA(dsDNA) and is the most commonly used DNA-binding dye for fluorescence quantitative PCR. In quantitative PCR, SYBR Green I can fluoresce after non-specific binding with double-stranded DNA (dsDNA), so the purpose of detecting the amplification of PCR products can be achieved by detecting the fluorescence intensity of SYBR Green I in the reaction system. In the free state, SYBR Green I emits weak fluorescence. Once it binds to dsDNA, its fluorescence increases by 1000 times. The total fluorescence signal from a reaction is proportional to the amount of dsDNA present, and increases with the increase of amplified product. Therefore, by detecting the fluorescence signal intensity in the PCR reaction solution, the target gene can be accurately quantified, and the melting temperature of the amplified target DNA fragment can also be determined.
Instructions for use:
When used, the PCR reaction mixture was configured and 10000×SYBR Green I concentrated solution was added to the PCR reaction system so that the final concentration was 0.5× (adjusted between 0.2× and 1×). The above operations are recommended on ice.
Note: ① Please refer to the instructions for using DNA polymerase for preparation of reaction solution and PCR amplification conditions.
② For the use of the Realtime PCR amplification instrument, please refer to the instructions of each instrument.
Note:
Effect of concentration on fluorescence PCR results
The concentration of SYBR Green I is a key factor to ensure the success of fluorescence quantitative PCR experiments. If the concentration of SYBR Green I is too low, the change in the fluorescence signal will be reduced, which means that the sample with low copy may not be detectable; At high concentrations, the PCR reaction will be inhibited and the efficiency of PCR reaction will be reduced. Therefore, in general, when using SYBR Green I, the concentration should be optimized according to the actual situation, and the final concentration of the reaction is between 0.2× and 1×.
Effect of magnesium ion concentration
The inhibitory effect of SYBR Green I on PCR reaction could be reduced by increasing the concentration of magnesium ion. We recommend that the concentration of magnesium ions in the fluorescent PCR reaction with SYBR Green I be 0.5 ~ 3mM higher than that in the ordinary PCR reaction without SYBR Green I."
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Sorry, there is no experimental images.
Related Products
Sorry, there is no more information.
Related Paper
DNA framework-engineered chimeras platform enables selectively targeted protein degradation
Click to check >>Author：Zhou Li, Yu Bin, Gao Mengqiu, Chen Rui, Li Zhiyu, Gu Yueqing, Bian Jinlei, Ma Yi
IF：16.6000
Publish_to：Nature Communications
PMID：37495569
Thermus thermophilus Argonaute-Based Isothermal Amplification Assay for Ultrasensitive and Specific RNA Detection
Click to check >>Author：Changjing Yuan, Jie Fang, Weiling Fu
IF：8.0080
Publish_to：ANALYTICAL CHEMISTRY
PMID：37195043
A portable and partitioned DNA hydrogel chip for multitarget detection
Click to check >>Author：Yi Guo, Wenxing Li, Runchi Zhang, Siyu Cao, Xiaoli Zhu, Guifang Chen, Chang Feng
IF：7.5170
Publish_to：LAB ON A CHIP
PMID：37139578
Performance of gravity-driven membrane filtration for treatment of low-temperature and low-turbidity water: Effect of coagulation pretreatment
Click to check >>Author：Kai Li, Min Han, Yuqi Xu, Jianlin Zhang, Tong Wei, Gang Wen, Tinglin Huang
IF：7.3400
Publish_to：Journal of Water Process Engineering
PMID：
A Cas12a-based platform combined with gold nanoparticles for sensitive and visual detection of Alternaria solani
Click to check >>Author：Hangyu Guo, Yaqin Zhang, Fange Kong, Chunxia Wang, Shanshan Chen, Jiasi Wang, Di Wang
IF：6.8000
Publish_to：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
PMID：37418936
A polyethylene glycol enhanced ligation-triggered self-priming isothermal amplification for the detection of SARS-CoV-2 D614G mutation
Click to check >>Author：Luxin Yu, Zibin Tang, Yuanzhong Sun, Hai Yi, Yuebiao Tang, Yangqing Zhong, Dongchun Dian, Yanguang Cong, Houqi Wang, Zhaoyang Xie, Suhui He, Zhangquan Chen
IF：6.5560
Publish_to：TALANTA
PMID：37244245
Development and evaluation of a centrifugal disk system for the rapid detection of multiple pathogens and their antibiotic resistance genes in urinary tract infection
Click to check >>Author：Nianzhen Chen, Gen Li, Yuying Si, Yangqin Ye, Tong Zhang, Dali Chi, Wenyan Zhang, Lifeng Pan, Guoying Qu, Yun Lu, Ming Zong, Guodong Sui, Lieying Fan
IF：6.0640
Publish_to：Frontiers in Microbiology
PMID：37200917
Chronic intermittent hypobaric hypoxia ameliorates osteoporosis after spinal cord injury through balancing osteoblast and osteoclast activities in rats
Click to check >>Author：Li Zhang, Yingchao Yin, Jialiang Guo, Lin Jin, Zhiyong Hou
IF：6.0550
Publish_to：Frontiers in Endocrinology
PMID：37229453
Baicalin Ameliorates Lung Injury in Rats by Inhibiting NLRP3 Inflammasome Activation via NF-κB Signaling Pathway
Click to check >>Author：Xingguan Yang, Jiahui Han, Zhirong Huan, Ce Xu, Qiubo Wang, Xin Ge
IF：6.0050
Publish_to：AMERICAN JOURNAL OF CHINESE MEDICINE
PMID：37036317
A molecular gated HRCA quick sensing system intelligently controlled by APE1
Click to check >>Author：Qi Kang, Xiaoyan Yang, Yumin Du, Yinxiao Qi, Zhiqiang He, Hua Xiang
IF：5.3040
Publish_to：MICROCHEMICAL JOURNAL
PMID：
Design,synthesis and anticancer evaluation of novel oncolytic peptide-chlorambucil conjugates
Click to check >>Author：Hao Yin, Xing-Yan Fu, Han-Yu Gao, Yan-Nan Ma, Jing-Fang Yao, Shan-Shan Du, Yun-Kun Qi, Ke-Wei Wang
IF：5.1000
Publish_to：BIOORGANIC CHEMISTRY
PMID：37331169
Adrenomedullin induces cisplatin chemoresistance in ovarian cancer through reprogramming of glucose metabolism
Click to check >>Author：Lei Dou, Enting Lu, Dongli Tian, Fangmei Li, Lei Deng, Yi Zhang
IF：4.9000
Publish_to：Journal of Translational Internal Medicine
PMID：37408575
UBP43 promotes epithelial ovarian carcinogenesis via activation of β-catenin signaling pathway
Click to check >>Author：Hongyang Xie, Junyu Chen, Changyan Ma, Jingjing Zhao, Manhua Cui
IF：4.4730
Publish_to：CELL BIOLOGY INTERNATIONAL
PMID：37186433
Dipicolinic acid enhances kiwifruit resistance to Botrytis cinerea by promoting phenolics accumulation
Click to check >>Author：Su-Yan Wang, Yi-Bo Pang, Yuan Tao, Xin-Chi Shi, Zhang Yun-Jiao, Yan-Xia Wang, Jiang Yong-Hui, Xin-Yu Ji, Bing-Lin Wang, Daniela D. Herrera-Balandrano, Pedro Laborda
IF：4.4620
Publish_to：PEST MANAGEMENT SCIENCE
PMID：37024430
SMURF1 inhibits the Th17 and Th17.1 polarization and improves the Treg/Th17 imbalance in systemic lupus erythematosus through the ubiquitination of RORγt
Click to check >>Author：Wei Zhong, Leheng Feng, Wei Tian, Hongbo Qu, Haibo Xu, Ke Ning, Li Liu, Wei Liu, Xiaowei Gong, Hong Chen
IF：4.1740
Publish_to：MOLECULAR IMMUNOLOGY
PMID：37054520
SIX1 induced HULC modulates neuropathic pain and Schwann cell oxidative stress after sciatic nerve injury
Click to check >>Author：Jinyuan Zhang, Zhi Mei, Wanxiang Yao, Chenyi Zhao, Shutong Wu, Jun Ouyang
IF：3.5000
Publish_to：GENE
PMID：37479098
Saltatory rolling circle amplification assay coupled with photosensitization method for rapid and sensitive detection of Salmonella in food
Click to check >>Author：Wang Huibin, Yang Qian, Xu Hui, Zhang Yunzhe, Lu Xin, Zhang Wei
IF：3.4980
Publish_to：EUROPEAN FOOD RESEARCH AND TECHNOLOGY
PMID：
Knockdown of Mmu-circ-0001380 Attenuates Myocardial Ischemia/Reperfusion Injury via Modulating miR-106b-5p/Phlpp2 Axis
Click to check >>Author：Wang Li, Wang Chuanhe, Sun Zhaoqing, Du Aolin, Shan Fei, Sun Zhijun
IF：3.4000
Publish_to：Journal of Cardiovascular Translational Research
PMID：37474690
Detection of Periodontal Pathogens Based on an Integrated Continuous Flow PCR and Capillary Electrophoresis Microfluidic Chip
Click to check >>Author：Bo Yang, Jiaxin Huang, Chunxian Tao, Zhenqing Li, Dawei Zhang, Yoshinori Yamaguchi
IF：3.3440
Publish_to：Separations
PMID：
Protein kinase C inhibitor chelerythrine attenuates partial unilateral ureteral obstruction induced kidney injury in neonatal rats
Click to check >>Author：Bo Shi,Shixing Li,Hao Ju,Xin Liu,Dan Li,Ying Li
IF：3.2340
Publish_to：Life Sciences
PMID：30439378
CircIFFO1 suppresses tumor growth and metastasis of cutaneous squamous cell carcinoma by targeting the miR-424-5p/NFIB axis
Click to check >>Author：Yu Hui, Liu Penglin, Chen Tianli
IF：3.0000
Publish_to：ARCHIVES OF DERMATOLOGICAL RESEARCH
PMID：37405427
Elevation of LEM Domain Containing 1 Predicts Poor Prognosis of NSCLC Patients and Triggers Malignant Stemness and Invasion of NSCLC Cells by Stimulating PI3K/AKT Pathway
Click to check >>Author：Li Li, Zhang Pei
IF：2.6160
Publish_to：CURRENT MOLECULAR MEDICINE
PMID：36967459
Inhibition of TMUB1 blocks apoptosis and NF-κB pathway-mediated inflammation in recurrent spontaneous abortion
Click to check >>Author：Xiuping Zhang, Yuanjing Hu, Zhiping Zhang, Xueluo Zhang, Lixia Liang, Xiangrong Cui, Yuanxia Wu, Fen Hu, Xueqing Wu
IF：2.4930
Publish_to：Immunity Inflammation and Disease
PMID：37249279
NLRP3 inflammasome activation in mesenchymal stem cells inhibits osteogenic differentiation and enhances adipogenic differentiation
Click to check >>Author：Linghao Wang,Ke Chen,Xinxing Wan,Fang Wang,Zi Guo,
IF：2.4060
Publish_to：Biochemical and Biophysical Research Communications
PMID：28167279
Downregulation of circ_0035292 Alleviates LPS-Induced WI-38 Cell Injury via Targeting miR-494-3p/TLR4 Pathway in Infantile Pneumonia
Click to check >>Author：Dai Zhenzhao, Hu Jiansheng, Luo Zhiying, Xiao Jianhua
IF：2.4000
Publish_to：BIOCHEMICAL GENETICS
PMID：37500967
Complete genomic sequence of turnip mosaic virus infecting passionfruit in Fujian province of China
Click to check >>Author：Li Xuefeng, Xie Lixue, Chen Xihong, Chen Jiangshan, Shen Jianguo, Gao Fangluan
IF：2.2000
Publish_to：JOURNAL OF PLANT PATHOLOGY
PMID：
ELK3 Targeting AEG1 Promotes Migration and Invasion of Ovarian Cancer Cells under Hypoxia
Click to check >>Author：Xiaoyu Yu, Chun Du, Yifei Cui, Yang Jiang, Di Feng
IF：2.0000
Publish_to：BIOLOGICAL & PHARMACEUTICAL BULLETIN
PMID：37394639
Effects of ELL-associated factor 2 on ultraviolet radiation-induced cataract formation in mice
Click to check >>Author：Yanhua Jiang, Rongrong Fu, Jiangyue Zhao, Di Wu, G
IF：1.9220
Publish_to：Molecular Medicine Reports
PMID：26328919
Technical Q&A
Service for Scientific Research, Responsible for the Life Science
Contact Us
Address: No 85, Liandong U Valley, Middle Zone, 101102, Tongzhou Dist,
Beijing, China
Tel: +86 010 50973105
Email: info@solarbio.com
Order Guide
Service Guarantee
Resources Download
Copyright © 2005-2021 Solarbio all rights reserved.
SYBR Green I (PCR级，10000X)和SYBR Green I (10000X)有什么区别 2022-08-01 09:00:08
Administrator您好，PCR级的灵敏性更好，可以用于荧光定量PCR实验中，另一个是用于电泳检测的