Malondialdehyde(MDA) Content Assay Kit

Product Description

Detection Device: Spectrophotometer
Application: Lipid peroxide is produced by the action of oxygen free radicals on unsaturated fatty acid, then resolves to compounds, including malondialdehyde (MDA), the level of lipid peroxidation can be showed by detecting the level of MDA.
Under acidic and high temperature conditions, the brown red 3,5,5- three methyl sulfamethoxazole -2,4-two ketone was synthesized with MDA and thiobarbituric acid (TBA) taking place condensation reaction, the largest absorption wavelength is 532 nm.The content of lipid peroxidation can be estimated after colorimetric. But the soluble sugar will disturb the detection, the production ( color reaction of soluble suger with TBA) have absorption wavelength in 450 nm and 532 nm. In this kit, the MDA content is calculated by the difference between the absorbance at 532 nm, 450 nm and 600 nm.
Due to the effect of sucrose in plant tissues and glucose in animal tissues, this kit have two computational formulas for sucrose and glucose. The two formulas are suit for fat class material.
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