α-amylase Activity Assay Kit

Detection Device :

Microplate reader

Application :

Amylase including α-amylase and β-amylase. α-amylase (EC 3.2.1.1) randomly catalyze the hydrolysis of α-1,4-glycosidic bonds in starch to produce reducing sugars such as glucose, maltose, maltotriose, dextrin, etc. At the same time, the viscosity of starch is reduced, so it is also called liquifying enzyme.
Starch hydrolase catalyzes the hydrolysis of starch to produce reducing sugar. 3,5-dinitrosalicylic acid is reduced to brown red substance by the reducing sugar. The brown red substance has absorption peak at 540 nm. The activity of amylase was calculated by measuring the increasing rate of absorbance at 540 nm. α-amylase is thermostable, but β-amylase could be passivated at 70℃ for 15 min. Therefore, only α-amylase could catalyze starch hydrolysis when the crude enzyme solution is passivated at 70℃ for 15 min.

Product Description :