Succinate Dehydrogenase(SDH) Activity Assay Kit

Detection Device :

Microplate reader

Application :

SDH (EC 1.3.5.1) is widely found in animals, plants, microorganisms and cultured cells. SDH is a marker enzyme of mitochondria. It is a membrane binding enzyme located in the inner membrane of mitochondria. It is one of the key points of respiratory electron transfer and oxidative phosphorylation. In addition, it provides electrons for the respiratory chain of various prokaryotic cells.
SDH can catalyze the dehydrogenation of succinic acid to fumaric acid. The dehydrogenation can reduce 2,6-dichlorophenol indophenol (DCPIP) under the transfer of phenazine dimethyl sulfate (PMS). 2,6-DCPIP has a characteristic absorption peak at 600 nm. The reduction rate of 2,6-DCPIP is determined by the change of absorbance at 600 nm , which represents the activity of SDH enzyme.

Product Description :