Uric Acid(UA) Content Assay Kit

Representative standard curve

Product Description

Detection Device: Microplate reader
Application: Uric acid is the end product of purine metabolism. Disorders of purine metabolism, energy metabolism and renal excretion of uric acid can cause the increase or decrease of plasma uric acid level. And then lead to a variety of diseases such as ventilation, kidney disease, cardiovascular disease. Therefore, the determination of uric acid content has an important guiding significance in clinical diagnosis.
Uricase can catalyze the decomposition of uric acid into allantoin, CO2 and H2O2. Then Fe2+ in potassium ferrocyanide is oxidized by H2O2 to form Fe3+. Fe3+ can further react with 4-aminoantipyrine and phenol to form red Quinones, which has a characteristic absorption peak at 505 nm. The content of uric acid can be calculated by measuring the absorbance value at 505 nm.
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