Recombinant Human C-C Motif Chemokine 2/CCL2/MCP-1

Description :

Recombinant Human C-C Motif Chemokine 2 is produced by our E.coli expression system and the target gene encoding Gln24-Thr99 is expressed.

Formulation :

Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH7.4.

Purity :

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin :

Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.

Reconstitution :

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 米g/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage :

Lyophilized protein should be stored at ＜ -20℃, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.
Aliquots of reconstituted samples are stable at ＜ -20℃ for 3 months.

Background :

The chemokine (C-C motif) ligand 2 (CCL2), also known as monocyte chemoattractant protein (MCP)-1 and small inducible cytokine A2 (SCYA2)), is a small cytokine that belongs to the CC chemokine family responsible for monocyte attraction. Its cognate receptor, CCR2, play a critical role in regulating nociceptive processes during neuropathic pain. Both CCL2 and CCR2 are implicated in induction of autoimmunity. CCL2 recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection. Recently research also showed that CCL2 might be useful as a biomarker of fibrosis as well as a target for therapeutic intervention.