Recombinant Human NPTX1

Description :

Recombinant Human Neuronal Pentraxin-1 is produced by our Mammalian expression system and the target gene encoding Gln23-Asn432 is expressed with a 6His tag at the C-terminus.

Formulation :

Lyophilized from a 0.2 米m filtered solution of PBS,1mM EDTA,PH7.4.

Purity :

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin :

Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Reconstitution :

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 米g/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage :

Lyophilized protein should be stored at < -20～C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7～C for 2-7 days.Aliquots of reconstituted samples are stable at < -20～C for 3 months.

Background :

Neuronal Pentraxin (NPTX1, NP1) is a secreted glycoprotein within the Pentraxin family. NPTX1 is co-expressed and forms heteromultimers with the related secreted protein, NPTX2/NARP, NPTXR (Neuronal Pentraxin Receptor) at excitatory synapses. Mature human NPTX1 shares 97% aa sequence identity with mouse, and rat NPTX1. It is produced by hippocampal, cerebral and cerebellar neurons, retinal ganglia and the inner nuclear layer of the retina. It is enriched on presynaptic axonal membranes where it forms complexes with NPTXR. It may be involved in mediating uptake of synaptic material during synapse remodeling or in mediating the synaptic clustering of AMPA glutamate receptors at a subset of excitatory synapses.