Anti-RAD1 Polyclonal Antibody

Conjugation :

Unconjugation

Host :

Rabbit

Clonality :

Polyclonal Antibody

Gene symbol :

RAD1

Immunogen :

Recombinant protein of human RAD1

Synonym :

HRAD1;REC1

Isotype :

IgG

Gene ID :

5810

Clone No :

Swiss Port :

O60671

Molecular weight :

8/28/32kDa

Purification :

Affinity purification

Reactivity :

Human Mouse Rat

Application :

IHC

Recommended dilution :

IHC 1:100-300.

Subcellular location :

Nucleus

Storage :

Store at -20～C. Avoid freeze / thaw cycles.

Background :

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.