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Soluble Acid Invertase (S-AI) Assay Kit
Soluble Acid Invertase (S-AI) Assay Kit
Cat.No:
BC2470
Brand:
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
BC2470-50T/24S Inquiry Inquiry Inquiry Inquiry
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测定意义:蔗糖转化酶(Invertase,Ivr)催化蔗糖不可逆地分解为果糖和葡萄糖,是高等植物蔗糖代谢关键酶之一。根据最适 pH,Ivr 分为酸性转化酶(AI)和中性转化酶(NI)两种类型。AI 的最适pH 为3~5。AI 分为可溶性AI(S-AI)和细胞壁不溶性AI(B-AI)两种类型。S-AI 主要存在于细胞液泡或自由空间中,最适 pH 为4.5~5.0(酸性),通过降解液泡中蔗糖,调节液泡中蔗糖的利用和果实内糖类的积累。
测定原理:S-AI 催化蔗糖降解产生还原糖,进一步与3,5-二硝基水杨酸反应,生成棕红色氨基化合物,在510nm 有特征光吸收,在一定范围内510nm 光吸收增加速率与S-AI 活性成正比。
自备用品:可见分光光度计、台式离心机、水浴锅、移液器、1mL 玻璃比色皿、研钵、冰和蒸馏水。

Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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