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Determination of Significance：
β-galactosidase (β-GAL, EC 3.2.1.23) is an enzyme found broadly in animals, plants, microorganisms and cultured cells, which can catalyze the hydrolysis of β-galactosyl bonds and also has the function of transglycosylation. β-GAL can release stored energy for the rapid growth of plants, also catalyzes the degradation of polysaccharides, glycoproteins, and galactose terminal galactose residues in normal polysaccharide metabolism, cell wall component metabolism and during aging cell wall to release free galactose.
Measurement Principle：
β-GAL can catalyze the p-nitrophenyl-β-pyran galactoside to p-nitrophenol. The product has characteristic of absorption at 400 nm. In this kit, the β-GAL activity is quantified by measuring the increase in the color development at 400 nm.
Self Provided：
Spectrophotometer, desk centrifuge, water baths/constant temperature incubators, ultrasonic cell disruptor, transferpettor, 1 mL glass cuvette, mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
