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Determination of Significance：
S-NR catalyzes the reduction of nitrate to nitrite in soil, which is the key enzyme of nitrate reduction in soil. Study on the activity of S-NR is of great significance for rational fertilization and reduction of nitrogen loss.
Measurement Principle：
S-NR catalyzes the reduction of nitrate to nitrite, NO3-+NADH+H+→NO2-+NAD++H2O; the generated nitrite can quantitatively generate red azo compounds with p-aminobenzenesulfonic acid and α-naphthylamine under acidic conditions; The unreacted NADH will inhibit the subsequent color reaction, and then carry out the subsequent reaction with PMS; the generated red azo compounds are 520 nm has a maximum absorption peak, which can be determined by spectrophotometry.
Self Provided：
Spectrophotometer, water bath, low temperature centrifuge, 1ml glass cuvette, distilled water, 30 mesh sieve (or smaller), ice and distilled water.
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