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Determination of Significance：
Soil protease is involved in the transformation of amino acids, proteins and other organic compounds containing protein nitrogen in soil. Those hydrolytic product is one of the nitrogen sources of higher plants. Soil neutral protease catalyzes the hydrolysis of protein in neutral environment, which is related to soil organic matter content, nitrogen and other soil properties.
Measurement Principle：
Under neutral conditions, soil neutral protease could hydrolyze casein to produce tyrosine. In alkaline conditions, tyrosine reduced phosphomolybdic acid compound to form tungsten blue with absorbance peak in 680 nm.
Self Provided：
Spectrophotometer/microplate reader, water bath, transferpettor, micro glass cuvette/96 well flat-bottom plate, methylbenzene, distilled water, 50 mesh sieve (or smaller).
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