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CoenzymeⅡ NADP(H) Content Assay Kit

Storage:Store at -20℃,6 months

CoenzymeⅡ NADP(H) Content Assay Kit
Cat.No:
BC1105
Brand:
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
BC1105-100T/48S Inquiry Inquiry Inquiry Inquiry
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Determination of Significance:

Coenzyme II NADP (H) is widely present in animals, plants, microorganisms and cultured cells. The determination of NADP+ and NADPH content can calculate the content of NADP (NADPH + NADP+) and the ratio of NADPH/NADP+. The changes are related to the pentose phosphate pathway and biosynthesis and resistance The oxidation reaction is closely related. The ratio of NADPH/NADP+ is not only one of the main signs of cell redox state, but also has an important regulatory role in PPP pathway, biosynthesis and antioxidant metabolism.

Measurement Principle:

The NADP+ and NADPH in the sample were extracted with acidic and alkaline extracts, respectively. NADPH reduces the oxidized thiazole blue (MTT) to formazan through the hydrogen transfer effect of PMS, and detects the absorbance at 570nm to determine the NADPH content. Use 6-phosphate glucose dehydrogenase to reduce NADP+ to NADPH to detect NADP+ content.

Self Provided:

Spectrophotometer/microplate reader, centrifuge, water bath, mortar/homogenizer, sonicator, adjustable pipette, micro glass cuvettes/96 well plates , ice, and distilled water.

Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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