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Determination of Significance：
Pectin lyase (EC4.2.2.10) is an important part of pectinase and catalyzes the elimination and cleavage of pectin molecular chain. The sources are relatively wide, mainly derived from microorganisms, which is of great significance in increasing fruit juice production in the food processing industry, and also has potential application value in reducing environmental pollution and energy consumption.
Measurement Principle：
Pectin lyase acts on the α-1,4 glycosidic bond in pectin, and generates unsaturated oligogalacturonic acid having an unsaturated bond between the reducing end C4 and C5. It has a characteristic absorption peak at 235nm. The increase in absorbance at 235 nm is measured to indicate the activity of pectin lyase.
Self Provided：
Spectrophotometer/microplate reader, centrifuge, constant temperature water bath, transferpettor, micro quartz cuvette/96 well UV plate, mortar/homogenizer/cell ultrasonic crusher, ice and distilled water.
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