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Chemical Synthesis

G1010 Giemsa Stain Solution(Working Solution)

实验简介:
Giemsa Stain Solution is composed of azure and eosin. The chemical properties of various cell components are different, and the affinity for various dyes is also different. Eosinophilic particles are basic proteins, which are combined with eosin and dyed pink; eosinophilic particles, such as nucleoprotein or lymphocyte cytoplasm, are acid substances, which are combined with basic dye methylene blue or azure and dyed purple blue; neutral particles are in isoelectric state and can be combined with cosin and methylene blue, which are dyed light purple. All kinds of components are distinguished because of their own characteristics and the combination of different substances in Giemsa Stain Solution to present different colors. The Giemsa Stain Solution prepared by our company is made of imported Giemsa dye. It can dye the nucleus to purplish red or blue purple, and the cytoplasm to pink, presenting a clear cell dyeing image under the light microscope. It is mainly used to show the difference of the shape and size of various blood cells in blood smear, with good staining effect, strong staining power and clear staining.
实验步骤:
取姬姆萨浓缩液1mL，姬姆萨稀释液9mL充分混匀，即可使用。
1.按常规方法制备血涂片，待血膜干后，用甲醇固定2-3min。制备的血涂片需厚薄适宜，分布均匀，以免影响染色结果。
2.将血涂片或骨髓涂片放置染色架上，滴加稀释好的染色液使其覆盖全部血膜，室温染色15-30min。
用自来水缓慢从玻片一端冲洗(注意勿先倒去染液或直接对血膜冲洗)，晾干后镜检。
注意事项:
1.为了您的安全和健康，请穿实验服并戴一次性手套操作。
2.如果染色过浓或者过淡，请自行调整染色时间或姬姆萨工作液浓度。
3.原液稀释后可能会出现少量沉淀，不影响使用；染色结束用水洗净即可。
4.姬姆萨原液采用常规方法配制并用滤纸过滤，请在使用时不要接触到水。否则时间长了会失效。
5.PH对细胞染色有影响。染色用载玻片必须清洁，无酸碱污染以免影响染色结果。
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