Product Introduction:

succinate dehydrogenase (SDH) is the first enzyme of the succinate oxidase family and is located in mitochondria. The optimal pH for this enzyme activity is 7.6-8.5. This enzyme is involved in the tricarboxylic acid cycle, and in histochemistry, this enzyme activity is often used as a representative of the tricarboxylic acid cycle, and is also used as one of the marker enzymes of the mitochondria. The tissues with high activity of this enzyme are myocardium, renal tubular epithelium and hepatocytes. The enzyme is sensitive to fixative, so fresh tissue sections are required.

Our Succinate Dehydrogenase Staining Kit (Tetrazolium Salt Method) uses succinate as the substrate, which is dehydrogenated under the action of the enzyme. nitroblue tetrazolium (NBT) is the hydrogen acceptor, which receives the hydrogen and is reduced to methoxylate, which is blue-purple in color and is used to represent the activity of succinate dehydrogenase.The NBT incubation solution contains a special intermediate hydrogen delivering agent, which allows for a more accurate localization and clearer staining.。

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