Succinate Dehydrogenase Stain Kit(Tetrazole Salt Method)
Cat.No：G2000 Solarbio
Storage：Store at 2-8℃，avoid light，6 months
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> Dye Reagents > Enzymatic Stain > Succinate Dehydrogenase Stain Kit(Tetrazole Salt Method)
Product Information
| Name | Succinate Dehydrogenase Stain Kit(Tetrazole Salt Method) |
| Storage | Store at 2-8℃，avoid light，6 months |
| Delivery Time | 2-3 days |
| Unit | Kit |
| Specification | 60ml |
Product Introduction:
succinate dehydrogenase (SDH) is the first enzyme of the succinate oxidase family and is located in mitochondria. The optimal pH for this enzyme activity is 7.6-8.5. This enzyme is involved in the tricarboxylic acid cycle, and in histochemistry, this enzyme activity is often used as a representative of the tricarboxylic acid cycle, and is also used as one of the marker enzymes of the mitochondria. The tissues with high activity of this enzyme are myocardium, renal tubular epithelium and hepatocytes. The enzyme is sensitive to fixative, so fresh tissue sections are required.
Our Succinate Dehydrogenase Staining Kit (Tetrazolium Salt Method) uses succinate as the substrate, which is dehydrogenated under the action of the enzyme. nitroblue tetrazolium (NBT) is the hydrogen acceptor, which receives the hydrogen and is reduced to methoxylate, which is blue-purple in color and is used to represent the activity of succinate dehydrogenase.The NBT incubation solution contains a special intermediate hydrogen delivering agent, which allows for a more accurate localization and clearer staining.。
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
小鼠肌肉新鲜冰冻切片
酶活性位点为蓝紫色沉淀，背景接近于无色。
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Partial inhibition of class III PI3K VPS-34 ameliorates motor aging and prolongs health span
Click to check >>Author：Zhongliang Hu, Yamei Luo, Yuting Liu, Yaru Luo, Liangce Wang, Shengsong Gou, Yuling Peng, Rui Wei, Da Jia, Yuan Wang, Shangbang Gao, Yan Zhang
IF：9.8000
Publish_to：PLOS BIOLOGY
PMID：37432924
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