ATPase Stain Kit(Calcium-Cobalt Method)
Cat.No：G2380 Solarbio
Storage：Store at 2-8℃，avoid light，6 months
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PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
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Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
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Spectrophotometer
Centrifuge
Shaker
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Product Information
| Name | ATPase Stain Kit(Calcium-Cobalt Method) |
| Storage | Store at 2-8℃，avoid light，6 months |
| Delivery Time | 2-3 days |
| Unit | Kit |
| Specification | 5*50ml |
Product Introduction:
Adenosine triphosphatase (ATPase) is a kind of hydrolase, which catalyzes the hydrolysis of ATP. According to different activators, inhibitors and enzyme location, ATPase can be divided into membrane ATPase, myosin ATPase and mitochondrial ATPase. ATPase can hydrolyze adenosine triphosphate into adenosine diphosphate and phosphoric acid. This enzyme only acts on the high energy bond between phosphoric acid and phosphoric acid, thus releasing a lot of energy. The catalytic reaction is as follows: A-P-P-P + H2O→A-P-P + H3PO4 + energy.
The principle of Adenosine Triphosphatase Staining Kit (Calcium-Cobalt Method) is that the reaction of myosin adenosine triphosphatase (mATPase) hydrolyzing adenosine triphosphate to adenosine diphosphate and phosphoric acid is pH-dependent, which in turn makes use of the effect of different pH on the activity of mATPase to perform the differentiation of myofibrillar fractional staining.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
肌肉冰冻切片
左半边为碱性孵育液染色结果，右半边为酸性孵育染色结果，强阳性位点着棕黑色近黑色，弱阳性位点着棕黄色，阴性位点着淡黄色或不着色。血管周边偶有强阳性非特异着色属正常现象。
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