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Product Introduction:
Adenosine triphosphatase (ATPase) is a kind of hydrolase, which catalyzes the hydrolysis of ATP. According to different activators, inhibitors and enzyme location, ATPase can be divided into membrane ATPase, myosin ATPase and mitochondrial ATPase. ATPase can hydrolyze adenosine triphosphate into adenosine diphosphate and phosphoric acid. This enzyme only acts on the high energy bond between phosphoric acid and phosphoric acid, thus releasing a lot of energy. The catalytic reaction is as follows: A-P-P-P + H2O→A-P-P + H3PO4 + energy.
The principle of Adenosine Triphosphatase Staining Kit (Calcium-Cobalt Method) is that the reaction of myosin adenosine triphosphatase (mATPase) hydrolyzing adenosine triphosphate to adenosine diphosphate and phosphoric acid is pH-dependent, which in turn makes use of the effect of different pH on the activity of mATPase to perform the differentiation of myofibrillar fractional staining.
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左半边为碱性孵育液染色结果，右半边为酸性孵育染色结果，强阳性位点着棕黑色近黑色，弱阳性位点着棕黄色，阴性位点着淡黄色或不着色。血管周边偶有强阳性非特异着色属正常现象。
