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Product Introduction:
Different amino acids have side chain groups with different chemical properties. Some have basic side chains, some have acid side chains, and the resulting proteins have different numbers of basic and acid groups. These groups will make proteins with different net charges in different pH solutions, and the whole protein molecule has more positive charges, that is, basic protein (isoelectric point tends to be alkaline); The whole protein has more negative charge, that is acid protein (isoelectric point tends to be acid)
The principle of Alkaline Fast Green Stain Kit is that basic protein combines with basic dye containing negative charge to show color. The most abundant basic protein in the cell is histone, which mainly exists in the nucleus. So after dyeing, most of the nucleus are dyed green. The staining solution is only used in scientific research field, and is not suitable for clinical diagnosis or other purposes.
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