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Your Position: Home > Protein and Immunity > Other Reagents > Western Blot > SDS-PAGE Gel Kit
SDS-PAGE Gel Kit

Storage:2-8℃, avoid light, valid for 1 year.

  p1200Manual
SDS-PAGE Gel Kit
Cat.No：
P1200
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
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Product Information Related Products Related Paper COA Technical Q&A



        25T

        50T

保存条件

30%制胶液(29:1)

100mL

100mL×2

2-8℃，避光      

1.5mol/LTris(pH8.8)

100mL

100mL×2

常温      

1.0mol/LTris(pH6.8)

30mL      

60mL      

常温      

PAGE胶凝固剂      

1g      

2g      

干粉2-8℃；溶液-20℃

10%SDS      

5mL

10mL      

常温      

PAGE胶促凝剂      

0.8mL

1.5mL

2-8℃，避光


产品简介：

本公司生产的SDS-PAGE凝胶制备试剂盒是按照经典方法配制而成。本试剂盒提供了配制SDS-PAGE凝胶所需的各种试剂，用户只需自备制胶器具和蒸馏水，即可配制PAGE胶。本试剂盒可配制不同规格、不同聚合度的PAGE胶(即聚丙烯酰胺凝胶) 约25/50块。

注意事项：

1. PAGE胶促凝剂和10%PAGE胶凝固剂最后加，在加入前需混匀前面所加试剂。10%PAGE胶凝固剂溶液在4℃有效期为一周； PAGE胶促凝剂易挥发，使用后请盖紧瓶盖。在常温下胶30分钟内可以凝固，如温度过低，可放37℃温箱凝固。灌完分离胶后，轻轻的加1mL ddH2O封上层，胶凝固后可见到分界线。灌浓缩胶前，先倾去水层，再用吸水纸吸干。浓缩胶灌好后立刻插入梳子。浓缩胶凝固后，放入电泳液中(让电泳液漫过加样孔)，轻轻的拨出梳子，可防加样孔变形。

2. 配制量可按上表等比加减。如果所用胶浓度与上面不同，可自行调整，主要是调整30% 制胶液的量（需要浓度×总体积/30%），最后用水补足总体积。

配胶说明：

1. 先在PAGE胶凝固剂干粉中加入蒸馏水或去离子水(每克PAGE胶凝固剂需加水10mL)配置成10%溶液，将溶液分装成小体积后冻存于-20℃，制备凝胶时融化后使用。4℃保存有效期为一周。检测SDS溶液是否澄清，若出现絮状物或沉淀可放37℃片刻至溶解，不影响效果。

2. 根据目标蛋白分子量大小，选取凝胶浓度，按下表配制。先配分离胶，再配浓缩胶。

         

分离胶12%          

分离胶8%        

1M Tris-HCl (PH6.8)          

0          

0          

         

2.5mL2.5mL2.5mL2.5mL最佳分离范围        

12-60 kD        

         

—          




Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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