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Alamar Blue reagent provides a simple, rapid, reliable and safe method for the detection of cell proliferation and cytotoxicity, which is suitable for high-throughput detection experiments. The main component of the assay reagent is a REDOX indicator. In the oxidation state,it showed a purplish-blue non-fluorescence, while in the reduction state, it changed to a reduction product with pink or red fluorescence, with an absorption peak of 530-560nm and a scattering peak of 590nm.
During cell proliferation, the ratio of NADPH/NADP, FADH/FAD, FMNH/FMN, and NADH/NAD in the cells increased and are in a reducing environment. The ingested dye is reduced by these metabolic intermediates and cytochromes and released outside the cell and dissolved in the medium, changing the medium from a non-fluorescent indigo blue to a fluorescent pink. Finally, a common spectrophotometer or a fluorescence photometer is used for detection, and the absorbance and fluorescence intensity are proportional to the number of viable cells.
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