Alamar Blue Cell Viability Assay Reagent
Cat.No：A7631 Solarbio
Storage：Store at 2-8℃，avoid light，1 year
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Oscillating Mixing & Stirring & Dispersing Instrument
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Product Information
| Name | Alamar Blue Cell Viability Assay Reagent |
| Storage | Store at 2-8℃，avoid light，1 year |
| Unit | Bottle |
| Specification | 1ml（100T) 5ml（500T) |
Alamar Blue reagent provides a simple, rapid, reliable and safe method for the detection of cell proliferation and cytotoxicity, which is suitable for high-throughput detection experiments. The main component of the assay reagent is a REDOX indicator. In the oxidation state,it showed a purplish-blue non-fluorescence, while in the reduction state, it changed to a reduction product with pink or red fluorescence, with an absorption peak of 530-560nm and a scattering peak of 590nm.
During cell proliferation, the ratio of NADPH/NADP, FADH/FAD, FMNH/FMN, and NADH/NAD in the cells increased and are in a reducing environment. The ingested dye is reduced by these metabolic intermediates and cytochromes and released outside the cell and dissolved in the medium, changing the medium from a non-fluorescent indigo blue to a fluorescent pink. Finally, a common spectrophotometer or a fluorescence photometer is used for detection, and the absorbance and fluorescence intensity are proportional to the number of viable cells.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
不同密度接种得细胞在同样的反应条件下呈现不同的颜色
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