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CAS:61926-22-5
Appearance:Solid
Storage:Powder : -20℃, 2 years;In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months
Purity:≥90%
Manual Download
Ethidium homodimer(EthD-I) is a high-affinity fluorescent nucleic acid dye that, when combined with DNA or RNA, can enhance fluorescence by more than 30 times. It is commonly used to stain mammals, bacteria, yeasts and fungi. EthD-I has a strong positive charge, so the dye cannot cross the cell membrane to stain living cells, but it can cross the disordered region of the dead cell membrane to reach the nucleus and embed the DNA double strand to produce red fluorescence. Therefore, EthD-I can accurately detect nucleic acid in solution or nucleic acid in disintegrated cells, and is a more sensitive nucleic acid stain.
Ex/Em (binding DNA) = 528/617 nm
How to use (for reference only)
Note:
The instructions apply to most cells, but different cell types, cell density, medium used, and other factors may affect the staining effect
1. Add 20 μL of 2 mM storage solution to 10 mL of sterile tissue culture-grade D-PBS and vortex thoroughly to make the final concentration of 4 μM (the recommended concentration is 0.1-10 μM, and the gradient setting for different cell lines is recommended to determine the optimal staining concentration).
2. Absorb 100-150μL of the above prepared working liquid and add it to the cell cap slide to completely cover it. Incubation is best done in a dish with a lid to prevent the dye from evaporating.
3. Incubate at room temperature for 30-45 min away from light. If the concentration of dyeing solution is too high or the temperature is too low, the incubation time can be appropriately reduced.
4. Add 10 μL D-PBS to a new microscope slide.
5. Using tweezers, carefully and quickly place the cover slide containing cells upside down on the slide containing D-PBS. In order to prevent the dye from evaporating, nail oil can be used to seal the slide.
6. The staining of cells was observed under fluorescence microscope.
