Tartrate-Resistant Acid Phosphatase(TRAP) Stain Kit
Cat.No：G1492 Solarbio
Storage：Store at -20℃，avoid light，3 months
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HIF
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> Dye Reagents > Enzymatic Stain > Tartrate-Resistant Acid Phosphatase(TRAP) Stain Kit
Product Information
| Name | Tartrate-Resistant Acid Phosphatase(TRAP) Stain Kit |
| Storage | Store at -20℃，avoid light，3 months |
| Unit | Kit |
| Specification | 4*10ml 4*20ml |
Product Introduction:
Acid phosphatase (ACP) is widely distributed in various tissues, mainly in the lysosome, so it is often used as a lysosome marker enzyme. The acid phosphatase outside of lysozyme exists in endoplasmic reticulum and cytoplasm. Acidic phosphatase is different in all kinds of animals. The optimum pH for its activity is 4.5-5.5. Tartrate-resistant acid phosphatase (TRAP), which mainly exists in normal human alveolar macrophages and leukemia human spleen cells, is released into the cell follicles rather than into the blood. The majority of TRAP in blood comes from osteoclasts, so we can know the function of osteoclasts by measuring TRAP in blood.
The reaction principle of Tartrate-Resistant Acid Phosphatase (TRAP) Stain Kit is basically the same as that of acid phosphatase staining. On the basis of conventional staining, tartrate resistant acid phosphatase was introduced for enzyme subtype screening. Only tartrate resistant acid phosphatase active sites were stained on cell or tissue sections, while conventional acid phosphatase sites were not stained. This staining solution can be used for fresh blood and cell smears, as well as for frozen sections and paraffin sections.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
小鼠肺石蜡/冰冻切片
阳性位点着紫红色，左侧为石蜡切片染色结果，右侧为冰冻切片染色结果，均为甲基绿复染
骨石蜡切片阳性位点着紫红色,细胞核着蓝紫色（苏木素衬染）
骨石蜡切片阳性位点着紫红色,细胞核着绿色（甲基绿衬染）
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