Products
HNB Indicator (100×, LAMP Grade)

Storage:Store at -20℃,avoid light,1 year.

HNB Indicator (100×, LAMP Grade)
Cat.No:
G1218
Brand:
Solarbio

LAMP是一种新型核酸扩增技术,采用4或6条能够识别目的基因上的6个特异区域的引物,依赖于Bst DNA聚合酶的强链置换活性,在30~60分钟内DNA扩增可达109~1010倍。LAMP检测方法分为多种,包括染料法、浊度法、电泳法及的TaqMan荧光探针法。

羟基萘酚蓝(HNB)是一种金属离子指示剂,HNB与镁离子(最适浓度一般为8mM-10mM)结合使得反应体系初始颜色为紫罗兰色,随着反应的进行,Mg2+与析出的焦磷酸根离子反应生成焦磷酸镁沉淀,羟基萘酚蓝失去了镁离子使得体系颜色变为天蓝色,而未反应的体系则仍保持着紫罗兰色。

Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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