Chlorophyll B

Examples of using this product（for reference only）

HPLC

HPLC analysis of chlorophyll and carotenoids were carried out using an Agilent 1260 instrument with a VWD detector . Samples (10 μL) were separated at 30℃ on a C18 column (150 mm × 3.9 mm i.d.; 4 μm particle size) using isopropanol and 80% acetonitrile–water at a flow rate of 0.5ml/min.

来源文献：Di H, Zhang Y, Ma J, Wei J, Wang Y, Li Z, Cui C, Fang P, Ma W, Li H, Sun B, Zhang F. Sucrose treatment delays senescence and maintains the postharvest quality of baby mustard (Brassica juncea var. gemmifera). Food Chem X. 2022 Mar 1;14:100272. doi: 10.1016/j.fochx.2022.100272. PMID: 35257095; PMCID: PMC8897633.

HPLC

HPLC analysis of carotenoids was carried out using an equipped with a variable wavelength detector (VWD). Samples (10 μL) were separated at 30℃ on a C18 column (150 × 3.9 mm id; 3 μm particle size) using isopropanol and 80% acetonitrile-water at a flow rate of 0.5 mL min?1 ; the absorbance was measured at 448 and 428 nm.

来源文献：Jian Y, Zhang C, Wang Y, Li Z, Chen J, Zhou W, Huang W, Jiang M, Zheng H, Li M, Miao H, Zhang F, Li H, Wang Q, Sun B. Characterization of the Role of the Neoxanthin Synthase Gene BoaNXS in Carotenoid Biosynthesis in Chinese Kale. Genes (Basel). 2021 Jul 24;12(8):1122. doi: 10.3390/genes12081122. PMID: 34440295; PMCID: PMC8393425.

HPLC

HPLC analysis of chlorophylls and carotenoids was carried out using an Agilent 1260 instrument with a variable wavelength detector (VWD) (Agilent Technologies, Inc., Palo Alto, USA).Samples (10 μL) were separated at 30 ℃ on a Waters C18 column (150 × 3.9 mm i.d.; 4 μm particle size) using isopropanol and 80% acetonitrile–water at a flow rate of 0.5 mL min?1. Absorbance was detected at 448 and 428 nm.

来源文献：Sun B, Di H, Zhang J, Xia P, Huang W, Jian Y, Zhang C, Zhang F. Effect of light on sensory quality, health-promoting phytochemicals and antioxidant capacity in post-harvest baby mustard. Food Chem. 2021 Mar 1;339:128057. doi: 10.1016/j.foodchem.2020.128057. Epub 2020 Sep 11. PMID: 32947106.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.