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Fura-FF pentapotassium salt
CAS:192140-58-2
Storage:Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity:≥95%
Appearance:Solid
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Among the ratiometric calcium indicators, Fura-2 and Indo-1 are most commonly used.
Fura-2 is excitation-ratioable while Indo-1 is emission-ratioable.?? Fura-2 is preferred for ratio-imaging microscopy, in which it is more practical to change excitation wavelengths than emission wavelengths.
Upon binding Ca2+, Fura-2 exhibits an absorption shift that can be observed by scanning the excitation spectrum between 300 and 400 nm, while monitoring the emission at ~510 nm.?? The cell-impermeant Fura-2FF is an analog of Fura-2 with much lower calcium binding affinity.
Remark：
These protocols are for reference only. Solarbio does not independently validate these
methods.
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