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Determination of Significance：
The presence of sulfhydryl allows proteins to undergo disulfide bond formation, thereby maintaining molecular stability and functionality. Sulfhydryl are also involved in redox reactions and have important biological roles. In cells, changes in sulfhydryl content are closely related to the onset and progression of a variety of diseases, and thus sulfhydryl have become an important object of study in the biomedical field. This kit determines the free sulfhydryl content of proteins.
Measurement Principle：
Under certain conditions, sulfhydryl group will undergo nucleophilic reaction, i.e. sulfhydryl group reacts with 5,5'-dithio-bis-nitrobenzoic acid (DTNB) to produce a yellow color compound with a maximum absorption peak at 412 nm, from which the free sulfhydryl content of protein can be calculated.
Self Provided：
Spectrophotometer/microplate reader, tabletop centrifuge, water bath, adjustable pipette, Micro glass cuvettes/96 well plates, acetone, mortar/homogenizer/ sonicator and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
