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My Cart> Antibodies > Primary Antibodies > Anti-Phospho-AKT(Ser473) Monoclonal AntibodyClone Type：Monoclonal Antibody
Storage：Store at -20℃.Avoid freeze/thaw cycles.
Gene ID：207
Purity：Phospho-AKT(Ser473)
Qty：
Size：
| Name | Anti-Phospho-AKT(Ser473) Monoclonal Antibody |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Dilution Ratio | WB 1:2000-10000. IHC 1:100-400. FCM 1X10^6 cells+0.5ug. |
| Target | Mouse |
| Background | Acts as a regulator of mitochondrial calcium uptake by mediating phosphorylation of MICU1 in the mitochondrial intermembrane space， impairing MICU1 maturation (PubMed:30504268).Acts as an inhibitor of tRNA methylation by mediating phosphorylation of the N-terminus of METTL1， thereby inhibiting METTL1 methyltransferase activity (PubMed:15861136).In response to LPAR1 receptor pathway activation， phosphorylates Rabin8/RAB3IP which alters its activity and phosphorylates WDR44 which induces WDR44 binding to Rab11， thereby switching Rab11 vesicular function from preciliary trafficking to endocytic recycling (PubMed:31204173). |
| Swiss Prot | P31749 |
| Gene ID | 207 |
| Subcellular Location | Cytoplasm Nucleus Cell membrane Mitochondrion intermembrane space |
| Immunogen | A synthetic peptide of human Phospho-AKT(Ser473) |
| Public Immunogen Range | 1-210aa |
| Calculated MW | 57/60/60kDa |
| Observed MW | Pig;Rabbit;Canine;Chicken;Zebrafish;Duck |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage | Store at -20℃.Avoid freeze/thaw cycles. |
| Purity | Phospho-AKT(Ser473) |
| Conjugation Concentration | bottle |
| Conjugation | 85-98kDa |
| Specification | 50ul 100ul |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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