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CAS:7660-25-5
Appearance:White to off-white powder
Storage:RT.
Purity:HPLC≥98%
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Nano LC-Orbitrap-MS/MS
Separation of peptides was performed on an Ultimate 3000RSLC nano system equipped with an RP-C18 guided column (2 μm, 2 cm×100 μm,) and an RP-C18 analytical column (2 μm, 150 mm×75 μm).Ultrapure water and acetonitrile containing 0.1% formic acid (FA) was used as solvent A and B, respectively, at a flow rate of 300 nL/min. The linear gradient program was: 0 min, 4% B; 6 min, 4% B; 50 min, 25% B; 58 min, 40% B；61 min, 85% B. The column was washed with 85% B for 5 min and re-equilibrated with 4% B for 7 min before the injection of next sample. The injection volume was 2.0 μL.The OrbitrapFusion mass spectrometer equipped with electron transfer dissociation (ETD) fragmentation mode was employed to the mapping of proteolytic peptides. The analytical parameters were: positive mode, mass range of 350-1800 m/z, MS resolution of 60,000, MS/MS resolution of 15,000, normalized collision energy of 30 eV.
References：
Zhang L, Xu L, Tu ZC, Wang HH, Luo J, Ma TX. Mechanisms of isoquercitrin attenuates ovalbumin glycation: Investigation by spectroscopy, spectrometry and molecular docking. Food Chem. 2020 Mar 30;309:125667. doi: 10.1016/j.foodchem.2019.125667. Epub 2019 Oct 19. PMID: 31679851.
HPLC
High-performance liquid chromatography (HPLC) with a NH2-RP column (4.6 × 250 mm, 5 μm) was used to determine the concentration of glucose and fructose together with the following parameters: injection volume = 10 μL, mobile phase = 75% acetonitrile, flow rate = 1.0 μL/min, and column heater temperature = 40℃.
References：
Han W, Wang Y, Li H, Diao S, Suo Y, Li T, Sun P, Li F, Fu J. Transcriptome and Metabolome Reveal Distinct Sugar Accumulation Pattern between PCNA and PCA Mature Persimmon Fruit. Int J Mol Sci. 2023 May 11;24(10):8599. doi: 10.3390/ijms24108599. PMID: 37239943; PMCID: PMC10217969.
HPLC
High-performance liquid chromatography (HPLC) with a NH2-RP column (4.6× 250 mm, 5 μm) was used to determine the concentration of glucose and fructose together with the following parameters: injection volume = 10 μL, mobile phase = 75% acetonitrile, flow rate = 1.0 μL/min, and column heater temperature = 40 ℃.
References：
Han W, Wang Y, Li H, Diao S, Suo Y, Li T, Sun P, Li F, Fu J. Transcriptome and Metabolome Reveal Distinct Sugar Accumulation Pattern between PCNA and PCA Mature Persimmon Fruit. Int J Mol Sci. 2023 May 11;24(10):8599. doi: 10.3390/ijms24108599. PMID: 37239943; PMCID: PMC10217969.
