CAS:57-48-7
Appearance:White to off-white powder
Storage:RT.
Purity:HPLC≥98%
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HPLC
The concentrations of D-allulose and D-fructose were determinedusing high-performance liquid chromatography (HPLC) on an LC-20A instrument equipped with an RID-20A refractive index detector and an NH2column (250 mm × 4.6 mm), operating at 30 ℃. The mobile phase consisted of 75 % acetonitrile and 25 % double distilled water (ddH2O), and the flow rate was set to 1.0 mL/min.
References:
Chen Y, Chen Y, Ming D, Zhu L, Jiang L. Highly efficiency production of D-allulose from inulin using curli fiber multi-enzyme cascade catalysis. Int J Biol Macromol. 2023 Jun 30;241:124468. doi: 10.1016/j.ijbiomac.2023.124468. Epub 2023 Apr 21. PMID: 37088188.
HPLC
HPLC analysis was performed on an LC20A HPLC system (Shimadzu, Japan) equipped with an SPD-20A ultraviolet detector and a C18 column (250 mm×4.6 mm, 5 μm, Shimadzu, Japan). The mobile phase was a mixture of 0.1 mol/L NaH2PO4-Na2HPO4 buffer (pH 6.7) and acetonitrile (83:17), and a flow rate of 1.0 mL/min was used. The wavelength of detection was 245 nm, and the column temperature was 30?C.
References:
Chen X, Sheng Z, Qiu S, Yang H, Jia J, Wang J, Jiang C. Purification, characterization and in vitro and in vivo immune enhancement of polysaccharides from mulberry leaves. PLoS One. 2019 Jan 2;14(1):e0208611. doi: 10.1371/journal.pone.0208611. PMID: 30601811; PMCID: PMC6314569.
![](http://solarbio.net/themes/ec36_01/slo/imgs/zhuyi.png)