Glucose-6-Phosphatase Stain Kit(Metal Precipitation Method)

Product Introduction:

Glucose-6-phosphatase (G-6-Pase) is a membrane-binding enzyme that exists in mammalian liver, kidney, intestine and other tissues. G-6-Pase is closely bound to microbodies and located in the endoplasmic reticulum. It is the main marker enzyme of endoplasmic reticulum. It can hydrolyze glucose-6-phosphate into glucose and phosphoric acid.G-6-Pase is a key enzyme in glycometabolism, which plays an important role in maintaining the relative constant blood sugar concentration. When blood sugar is lowered, G-6-Pase promotes glycogen conversion from liver to blood sugar. When lacking G-6-Pase, it will cause glycogen decomposition disorder and accumulate glycogen in liver, kidney, heart and other parts, leading to glycogen storage disease. The optimum pH of G-6-Pase is 6.5, which is also acceptable at pH 6.0-8.0. The most stable pH of G-6-Pase is 8.0, which is changeable at pH 5.0. PH 6.5-6.7 is commonly used in histochemical reactions.
Glucose-6-Phosphatase Stain Kit(Metal Precipitation Method) used heavy metal traps and phosphoric acid binding to show the activity of the enzyme. The enzyme is sensitive to immobilization and G-6-Pase is completely inhibited after paraffin-embedded tissues are immobilized in 80% acetic acid solution. Enzyme also become inactivated after short-term formaldehyde immobilization. It is recommended to use cryostatic section of fresh tissue, which can be fixed by acetone at low temperature for a short time, but not in general.

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Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

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