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CAS:482-36-0
Appearance:Light yellow to yellow powder
Storage:2-8℃
Purity:HPLC≥98%
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HPLC
HPLC analysis was performed using HPLC equipped with a C18 250 mm × 4.6 mm, 5 μm column . The analysis was maintained at a column temperature of 30℃with an injection volume of 10 μL at a flow rate of 1.0 mL/min. The mobile phase A was methanol and mobile phase B was ultrapure water containing 0.1% (m/v) formic acid. The gradient elution was performed as follows: 0–5 min, B 95–90%, 5–30 min, B 90–80%, 30–45 min, B 80–76%, 45–60 min, B 76–61%, 60–70 min, B 61–57%, 70–80 min, B 57–53%, 80–85 min, B 53–95%, 85–5 min, B 95%. The separated phenolic components were monitored at a wavelength of 280 nm.
来源文献：Zhang X, Li Y, Li Y, Zhao J, Cheng Y, Wang Y, Guan J. Changes of Bioactive Components and Antioxidant Capacity of Pear Ferment in Simulated Gastrointestinal Digestion In Vitro. Foods. 2023 Mar 13;12(6):1211. doi: 10.3390/foods12061211. PMID: 36981138; PMCID: PMC10048753.
UHPLC
Chromatographic separation of SPLFA and SPLF was done on an Agilent 1290 UHPLC system coupled to an auto sampler, a diode array detector (DAD) and an ACQUITY UHPLC? HSS T3 column (2.1 ×100 mm, 1.8 μm; Waters, Milford, MA, USA)maintained at 30 ℃. Eluent A was 0.05% formic acid in water and eluent B was 0.05% formic acid in acetonitrile. Elution of the SPLPA compounds was achieved using the following linear gradient elution (in %B): 0 min, 5%; 20 min, 60%; 20.1 min, 5%; 30 min, 5%, and for the SPLF compounds: 0 min, 20%, 25 min, 50%, 25.1 min, 20%, 35 min, 20%. The injection volume was 5 μL, with a flow rate of 0.3 mL/ min. All sample solutions were filtered through a 0.22 μm PTFE filter (Agilent Technologies) before analysis. The detection wavelength was 320 nm for SPLPA and 254 nm for SPLF.
来源文献：[1] Luo D , Mu T , Sun H .Profiling of phenolic acids and flavonoids in sweet potato (Ipomoea batatas L.) leaves and evaluation of their anti-oxidant and hypoglycemic activities[J].Food Bioscience, 2020, 39(44):100801.DOI:10.1016/j.fbio.2020.100801.
UPLC
The standards arbutin, (+)-catechin, (?)-epicatechin, neochlorogenic acid, cryptochlorogenic acid, chlorogenic acid, rutin, oleanolic, ursolic, p-coumaric, caffeic and quinic acid were purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Luteoloside, kaempferol-3-O-rutinoside, isorhamnetin-3-O-rutinoside, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C and hyperoside were bought from Beijing Solarbio Science and Technology Co., Ltd. (Beijing, China).
来源文献：Sun L, Tao S, Zhang S. Characterization and Quantification of Polyphenols and Triterpenoids in Thinned Young Fruits of Ten Pear Varieties by UPLC-Q TRAP-MS/MS. Molecules. 2019 Jan 3;24(1):159. doi: 10.3390/molecules24010159. PMID: 30609827; PMCID: PMC6337724.
