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My CartReactivity：Mouse,Monkey,Hamster,Rat,Human
Application：ICC,IP,IF,IHC,WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：11335
Host：Mouse
Qty：
Size：
| Name | Anti-CBX3 Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 5G10-F7-A12 |
| Dilution Ratio | WB 1:500-1000. IHC 1:50-100. IF 1:50-200. IP 1:20. ICC 1:50-200. |
| Target | CBX3 |
| Background | At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.[provided by RefSeq, Mar 2011] |
| Swiss Prot | Q13185 |
| Gene ID | 11335 |
| Subcellular Location | Nucleus |
| Immunogen | Recombinant protein of human CBX3 |
| Calculated MW | 21kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Monkey Hamster Rat Human |
| Application | ICC IP IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with CBX3 antibody diluted at 1:1000;Lane: CHO-K1,COS7,C6,3T3,HeLa.
Immunohistochemistry of paraffin-embedded Human prostate cancer with CBX3 antibody diluted at 1:100.
Immunofluorescence analysis of HeLa cells using CBX3 antibody diluted at 1:100.
Immunoprecipitation analysis of HeLa extracts using CBX3 antibody(IP:1:20;WB:1:1000).Control IgG: Normal Mouse IgG.
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